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KMID : 0368419890320040215
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Journal of Plant Biology
1989 Volume.32 No. 4 p.215 ~ p.226
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Effects of Ca^2+ and Polyamine on Callose Contents in Carrot Suspension Cultured Cells
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Abstract
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The effects of Ca^2+ on polyamines on callose contents of carrot suspension cultured cells were studied. The regeneration process of the cell wall of carrot protoplast observed through the electron microscope.
Treatment of the carrot suspension cultured cells with Ca^2+ and polyamines resulted in considerable increase on callose contents at 0.1 mM of Ca^2+ and polyamines, particulary spermidine. Poly-L-lysine and poly-L-ornithine increased about 30% and 100% of callose contents than that of the control respectively, whereas verapamil and flunarizine markedly decreased the callose contents. These effects of Ca^2+ and polyamines may be ascribed to a polycationic nature on the callose contents. Futhermore, Ca^2+ and calmodulin increased the callose contents as Ca^2+ of free ion rather than as Ca^2+ -calmodulin complex. During the cultivation of the protoplast, the regeneration of the cell wall was somewhat observed on the 4th day, however, it was inhibited by verapamil. These results suggested that the promotive action of Ca^2+ and polyamines were manifested in the callose contents and the regeneration of the cell wall.
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